FIG 3.

Modulation of humoral immune response of different antigens by PLG adjuvantation. (A) Mice were immunized intraperitoneally with individual antigens (20 μg of Ag85B, BP26, or PA) or with PLG (1 mg) or alum (200 μg) in a volume of 0.2 ml, and antigen-specific antibodies in the sera were measured by ELISA 2 weeks after the final immunization. (B to D) Antigen Ag85B (B)-, BP26 (C)-, and PA (D)-specific total IgG and subtype (IgG1, IgG2a, and IgG2b) titers were measured in individual mice (n = 5). One-way ANOVA followed by Tukey's multiple-comparison test was employed for calculating the significant difference. *, P < 0.05; **, P < 0.01; ***, P < 0.001. n.s, nonsignificant.