FIG 4.

Modulation of cytokine response of different antigens by adjuvantation with PLG. Three weeks after the last immunization, splenocytes were isolated and cultured in vitro with the respective antigens for 72 h, and release of antigen-specific cytokines was measured in the culture supernatant by ELISA. (A to C) Antigen Ag85B (A)-, BP26 (B)-, and PA (C)-specific cytokines IFN-γ, TNF-α, and IL-4 were measured in individual mice (n = 3). One-way ANOVA followed by Tukey's multiple-comparison test was employed for calculating the significant difference. *, P < 0.05; **, P < 0.01; ***, P < 0.001. n.s, nonsignificant.