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. 2018 Sep 21;86(10):e00537-18. doi: 10.1128/IAI.00537-18

FIG 5.

FIG 5

Modulation of immune response against antigen ESAT-6 by PLG adjuvantation. (A) C57BL/6J mice were immunized intraperitoneally with 20 μg of antigen ESAT-6 either alone or in the presence of PLG (1 mg) or control adjuvant DDA-MPL (250:25 μg). Antigen ESAT-6-specific total IgG and isotypes were measured in the serum samples of individual mice (n = 10) by ELISA. (B) Titers of total IgG and its isotypes, IgG1, IgG2a, and IgG2b, 2 weeks after the final immunization. (C) Three weeks after the last immunization, splenocytes were isolated and cultured in vitro with antigen ESAT-6 for 72 h, and release of IFN-γ, IL-2, TNF-α, IL-4, IL-6, IL-10, and IL-17A was measured in the culture supernatant. The mouse group D-M indicates the adjuvant DDA-MPL. One-way ANOVA followed by Tukey's multiple-comparison test was employed for calculating the significant difference. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. n.s, nonsignificant.