FIG 4.

C. burnetii T4BSS blocks IL-17-stimulated secretion of CXCL2/MIP-2 and CCL5/RANTES. The results of ELISA quantitation of CXCL2/MIP-2 (A) and CCL5/RANTES (B) protein levels after IL-17A (100 ng/ml) treatment of mock-infected, WT-infected, or dotA mutant-infected macrophages at 24 hpi are shown. The means ± SEM from three individual experiments performed in duplicate are shown. P values were determined by one-way ANOVA with Tukey's post hoc test and represent statistically significant differences compared to mock-infected cells or between WT and dotA mutant-infected cells. *, P < 0.05; **, P < 0.01; ****, P < 0.001.