FIG 6.

Flow cytometric analysis of macrophages from lung and nasal lavage fluid of mice immunized with aP titrations or aPs supplemented with RTX. (A to D) Percentages of macrophages (defined as CD-11b⁺ F4/80⁺) from live, single cells from nasal lavage fluid (A and B) and single-cell suspensions prepared from lung homogenate (C and D). Results shown are means ± SEM. **, P < 0.01; *, P < 0.05. P values were determined by one-way ANOVA with Tukey's post hoc test compared to the PBS (naive) group of challenged mice. Brackets between two points represent unpaired two-way t test.