FIG 2.

Binding of LprG to TLR2 is required to form TLR2-TLR1 complexes. SPR was used to test the abilities of TLR1 and TLR2 to form homodimers or heterodimers in the presence or absence of LprG or PIM6. TLR1 (A, C, D, F, G, and I) or TLR2 (B, E, and H) was immobilized on a CM5 sensor chip. (A to C) TLR1 (A) or TLR2 (B and C) was injected (100 nM) in the absence of a TLR2 agonist. (D to I) Alternatively, LprG (5 μM) or PIM6 (5 μM) was incubated with TLR1 (500 nM) (D and G) or TLR2 (500 nM) (E, F, H, and I) in a total volume of 150 μl HBSN buffer for 2 h at 37°C prior to sample injection. The results are representative of three independent experiments (A to C) or two independent experiments (D to I).