FIG 3.

ManLAM induces only minimal levels of ERK phosphorylation and IκBα degradation. BMDMs from wild-type or Tlr2^−/− mice were incubated for 15 min with 30 nM LprG or ManLAM at the indicated concentrations. The cells were lysed and analyzed by Western blotting to detect IκBα degradation and ERK phosphorylation.