FIG 5.

TLR2 and TLR1 are both required for agonist activity of LprG, LM, PILAM, and PIM6. THP1 cells were preincubated for 30 min with or without antibodies (10 μg/ml) as indicated and then incubated for 6 h in the continuous presence or absence of antibodies with LprG (A), LM (B), ManLAM (C), PILAM (D), or PIM6 (E) (all at 125 nM). TNF-α production was determined by ELISA. The results are representative of three independent experiments. The data shown represent the means ± SEM of triplicate assays. ****, P < 0.0001; ***, P < 0.001; **, P < 0.01; *, P < 0.05; ns, P > 0.05.