FIG 3.
Phenotypic characterization of clinical and genetically manipulated strains from patient 53. (A) Exoprotein profiles of strains grown in TSB for 5 h. Extracts were prepared from culture supernatants and analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Coomassie blue staining. M, protein ladder. (B) Intoxication of primary human neutrophils (hPMNs) with culture filtrates from the indicated S. aureus strains and controls (USA300 LAC wild type and agr mutant) as a percentage (vol/vol). Results represent the standard errors of the means (SEM) of data from 5 donors and 2 independent colonies. (C) Survival among mice infected with the indicated strains via intravenous inoculation (1 × 108 CFU). Mouse survival results are for 15 mice per group. P values for differences in survival were determined by Bonferroni-corrected log rank (Mantel-Cox) tests (P = 1.03 × 10−1 for agr-defective LAC versus agr+ LAC, P = 1.20 × 10−3 for the agr-defective nares strain versus the agr+ nares strain, P = 1.92 × 10−2 for the agr-defective blood strain versus the agr+ blood strain, P = 1 for the agr-defective nares strain versus agr-defective LAC, P = 1.80 × 10−3 for the agr-defective blood strain versus agr-defective LAC, and P = 2.40 × 10−3 for the agr-defective blood strain versus the agr-defective nares strain).