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. 2018 Oct 17;6:496. doi: 10.3389/fchem.2018.00496

Table 1.

Different detection methods of uric acid from biofluids (Gochman and Schmitz, 1971; Kageyama, 1971; Kabasakalian et al., 1973; Inoue et al., 2003; Perelló et al., 2005; Cooper et al., 2006; Arora et al., 2009; Uricase/PAP test, 2009; Bhawna and Pundir, 2010; Rocha and Rocha, 2010; Amir et al., 2011; Sanchez et al., 2011; Sidorova and Grigoriev, 2012; Hamzah et al., 2013; WitkowskaNery et al., 2016).

Principle Technique Biofluid sample Detection range (ppm) References
Enzymatic Uricase Method with 4-Aminodiphenylamine Diazonium Sulfate Spectrophotometric Urine 84–2185 Hamzah et al., 2013
Enzymatic Uricase Method with 4-Aminoantipyrine 1 mmol/lPeroxidase Spectrophotometric Urine, Serum 0–303 Uricase/PAP test, 2009
Enzymatic Uricase-Catalase Method Spectrophotometric Urine, Serum 0–319 Kageyama, 1971
Enzymatic Uricase-Peroxidase Method coupled with 3-methyl-2-benzothiazolinone hydrazone and N,N-dimethylaniline Spectrophotometric Serum 0–160 Gochman and Schmitz, 1971
Enzymatic Uricase Method with Tribromophenol-Aminoantipyrine Chromogen Spectrophotometric Serum 0–319 Kabasakalian et al., 1973
Reduction of Potassium ferricyanide and formation of Prussian blue Spectrophotometric Urine, Serum 0–185 Rocha and Rocha, 2010; Sanchez et al., 2011
Reduction of Cu(II) ion and complexation with 4,4′-Dicarboxy-2,2′-Bichinoline (BCA) Spectrophotometric Urine 2–17 Amir et al., 2011
Biosensor based on Uricase bound PVC membrane Enzyme-membrane biosensor Serum 0–100 Arora et al., 2009; Bhawna and Pundir, 2010
capillary zone electrophoresis Capillary Electrophoresis Urine 42–168 Sidorova and Grigoriev, 2012
Electrochemical detection Electrochemical Proof of concept 17-200 WitkowskaNery et al., 2016
high-performance liquid chromatography Chromatographic Saliva, Serum, Urine 1–27 Jen et al., 2002; Inoue et al., 2003; Perelló et al., 2005; Cooper et al., 2006