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. 2018 Oct 22;9:2490. doi: 10.3389/fmicb.2018.02490

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Copyright © 2018 Li, Sharp, Ataeian, Strous and de Beer.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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An example of extracellular carbonic anhydrase (eCA) activity measurement with membrane inlet mass spectrometry (MIMS), performed in the dark. X-axis is time, Y-axis represents the log enrichment of ¹³C¹⁸O¹⁸O, calculated with Equation (1). At the time point indicated by the dotted line, biomass was injected. S₁ and S₂ depict the slopes of linear regressions performed on the rate of decay of log enrichment of ¹³C¹⁸O¹⁸O before and after biomass injection.