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Primary mouse astrocytes have high basal SA-β-gal activity and demonstrate sensitivity to PQ. Representative images of primary mouse astrocytes stained for SA-β-gal activity. Astrocytes were treated with PQ (0, 15, 31, and 62.5 μM) for 24 h and fixed 5 days later. Astrocytes show high levels of SA-β-gal activity (left most panel) and show sensitivity to PQ, such that no cells remain when astrocytes were treated with 62.5 μM PQ for 24 h (right most panel).
