Figure 2.

Expression of HcPT1.2 in pure culture and ectomycorrhizal symbiosis with Pinus pinaster under P-sufficient and -limiting conditions. (a,b) Expression of HcPT1.2 in pure culture was determined by RT-qPCR on two-week-old mycelia grown in liquid medium supplemented with 1 mM of Pi (Day 15 Control), and then in mycelia transferred in a Pi-free medium during 24h (a) or 5 days (b). Mycelia were finally transferred in a Pi-rich medium for 24h (1 mM, P resupply). (c) Expression of HcPT1.2 in symbiotic condition was determined in extra-radical (Soil-growing hyphae) or root-associated hyphae (Ectomycorrhizal hyphae), obtained in rhizoboxes on thin soil layers enriched in P (+ P) or not (-P). No difference was recorded in any conditions. Values were normalized with housekeeping gene (HcTub). Data are means ± standard deviation (n = 4).