Figure 3.

Immunolocalization of HcPT1.2 in Hebeloma cylindrosporum hyphae forming ectomycorrhizas with Pinus pinaster under P-sufficient and -limiting conditions. Ectomycorrhizas were obtained in rhizoboxes on thin soil layers enriched in P (a,b) or not (c,d). Cross-sections labelled with secondary antibodies only show no signal (E). Images were obtained by confocal microscopy on 50 µm cross-sections labelled with HcPT1.2 primary antibodies and Dylight 405-conjugated secondary antibodies. m, hyphal mantle; exh, extra-radical hyphae; Hn, Hartig net; cc, cortical cell.