Figure 1.

Experimental setup of both hardware and communication of coupling light scattering with purification system.

a). The MALS detector was connected in-line, downstream of the purification unit. HIC purified samples were also sampled downstream of the in-line MALS by on-line UHPLC. On-line UHP-SEC-µMALS served as another application to monitor the fraction and molar mass of aggregates. The samples were separated by analytical SEC and evaluated using a UHP-SEC capable multi-angle light scattering detector (µMALS) and UV signal of the on-line UHPLC as the concentration source. b) The start or inject signal and the UV signal from the purification unit were sent to the in-line MALS via an I/O Box. The UV signal of the purification unit was the concentration source used to calculate M_w of the protein eluted from the HIC column. Real time molecular weight and start/stop trigger signals were sent by the in-line MALS detector to the purification system (via a voltage signal) for fractionation when the measured M_w fell between preset M_w ranges.