Figure 2.

Real time MALS data from a HIC purification.

a). The concentration and M_w signals are plotted along with the trigger signals. Throughout the purification, there is an increase in M_w (—) as aggregates saturate the HIC column and dimers coelute with the monomers. The protein is eluted from the column and passes through both the UV and in-line MALS detectors. The M_w is calculated in < 1 s using both the UV and light scattering signal and a start/stop trigger (- - -) is sent to the purification unit when the protein meets the preset M_w criteria. The concentration is calculated from the UV detector of the purification unit (---). b) No significant differences were observed between the data from real time (—) and post processed modes of analysis (---).