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. 2018 Sep 19;10(7):945–950. doi: 10.1080/19420862.2018.1505178

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© 2018 The Author(s). Published by Taylor & Francis Group

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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Figure 3. — On-line UHP-SEC-µMALS data correlates well with the real time M_w data from in-line MALS.

a) Real time molecular weight (—) shown previously is rescaled to show the increase in M_w of ~ 2750 Da. The HMW content (▲) increases by 1.5% over the course of the purification as determined by on-line UHP-SEC-µMALS. b) On-line UHP-SEC chromatograms of the samples at the start and stop trigger points showed an increase in aggregate content of 1.5%. On-line UHP-SEC-µMALS data confirmed the HMW content to be dimeric (inset). The 1.5% dimeric content correlates to a M_w increase of mAb by ~ 2250 Da. The real time data showed an increase of ~ 2750 Da and is within 0.5% dimeric content of the on-line UHP-SEC-µMALS data.