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Analysis of anti-WTA antibody binding to S. aureus. Protein A-deficient USA300 S. aureus were incubated with 125I-labelled anti-WTA antibodies and subjected to immunoradiometric assay and saturation binding analysis to determine antibody affinity. Titration curves are shown for 4462 (A), 6078 (B), 4497 (C) and 7578 (D), along with calculated KD values and estimated number of sites/bacterium.
