Figure 1.
Expression of Pp_phot:GFP in transgenic Arabidopsis (a) Schematic illustration of the binary vector construct used to make transgenic Arabidopsis expressing Pp_phot:GFP fusion protein. CaMV 35S Pro: cauliflower mosaic virus 35S promoter; Nos Ter: nopaline synthase terminator; Kmr: kanamycin resistant gene cassette(b) Immunoblot analysis of Pp_phot:GFP protein. Crude protein extract (30 μg) prepared from dark-grown transgenic Arabidopsis seedlings expressing Pp_phot:GFP (photA1:GFP-21, photA2:GFP-16, photB1:GFP-13, or photB2:GFP-6) was probed with the anti-GFP antibody. An asterisk on the left indicates a nonspecific band that had cross-reacted with GFP antibody. After chemiluminescence detection, the polyvinylidene difluoride (PVDF) membrane was stained with Coomassie brilliant blue (CBB) to visualize the amount of protein loaded in each lane (CBB stain).