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. 2018 Oct 23;13:6751–6767. doi: 10.2147/IJN.S174953

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© 2018 Song et al. This work is published and licensed by Dove Medical Press Limited

The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

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Histological analysis of the decalcification samples around Ti (A, B, E, F) and ICA-loaded (C, D, G, H) implants was evaluated by H&E (A–D) and Masson (E–H) staining. Histological morphology analyses based on H&E staining after 2 weeks implantation was expressed as was BF % and shown in I.

Notes: B, D, F and H depict zoomed areas of black box in A, C, E and G, respectively. Bars indicate 50 µm (A, C, E, G) and 20 µm (B, D, F, H). The black arrow denotes new bone formation; white arrow denotes osteoblast cell. Data are expressed as mean ± SD (n=3). *A statistical significance compared to the Ti group (P<0.05).

Abbreviations: BF%, area percentage of bone formation; ICA, icariin; Ti, titanium.