Figure 7.

Association of Sco1p and Coa6p with Cox2p. A, the different strains were grown to early stationary phase in 2% YPGal with a final 2-h growth in the presence of 2 mg/ml chloramphenicol. Mitochondria were labeled for 20 min and fractionated as in Fig. 2A. The digitonin extracts and eluates from the protein C antibody beads were separated by SDS-PAGE on a 12% polyacrylamide gel containing 6 m urea and 10% by weight glycerol. B, an enlargement of the Cox2p and cytochrome b region of the gel shown in A. C, the different strains of yeast were grown and mitochondria were labeled and fractionated, and the digitonin extracts and eluates from the protein C antibody beads were electrophoresed as in A. D, an enlargement of the Cox2p and cytochrome b region of the gel shown in C. E, the indicated strains were grown and mitochondria were labeled and fractionated, and the digitonin extracts electrophoresed as in A. F, the digitonin extracts of E were purified on protein C antibody beads and separated by BN-PAGE on a 4–13% polyacrylamide gel.