Figure 7.

Cytotoxicity profiling of AVG-233. A, assessment of cell metabolic activity after 48-h exposure of primary HBTECs to AVG-233. Assays were conducted in the presence of standard media, or RPMI supplemented with glucose or galactose as carbohydrate source to suppress glycolysis-induced masking of mitochondrial toxicity. B, multiplexed mitochondrial ToxGlo assay to determine cell membrane integrity and mitochondrial function after HBTEC exposure to AVG-233. C, in-cell ELISA determining effects on mitochondrial biogenesis after 48-h exposure to AVG-233. Symbols in A–C represent individual biological repeats (n = 3 each or greater); error bars, S.D.