Figure 6.
SPR sensorgrams of the interaction between lipid vesicles captured on a L1 chip and the engineered protein chimeras, IDFAPP2GLTP and IDGLTPFAPP2, versus GLTP and FAPP2–GLTPH. IDFAPP2 and IDGLTP are the ID loops of FAPP2 and GLTP, respectively. Vesicles are composed of POPC, 1-palmitoyl-2-oleoyl-sn-phosphatidylcholine, mixed with ganglioside GM1 (a–d) or 24:1 sulfatide, SF (e–h) in mol% ratios 100:0 (black), 95:5 (red), 90:10 (green), or 80:20 (blue). Proteins are dissolved at 0.1 mg/ml (5 μm) in running buffer (50 mm Tris-HCl, 150 mm NaCl, 1 mm EDTA, 1 mm DTT, pH 7.0). The flow rate is 5 μl/min. Red and black arrows indicate the start of protein injections and switching back to the buffer wash, respectively.