Figure 2.
The CARD9–CARD binds Zn2+ with a picomolar dissociation constant. Representative competition binding curves are shown for CARD9–CARDWT, CARD9–CARD mutants, or EDTA against mag-fura-2 (A) or indo-1 (B). Error bars represent the standard deviation of three technical replicates. Solid lines represent best fits to the generalized competition equation described by Wang (28). C, dissociation constants determined via competition against either mag-fura-2 or indo-1 as indicated. Error bars represent asymmetric profile likelihood 95% CI. For H73A and C10A, no upper limit was found for the 95% CI. For EDTA, either in competition with mag-fura-2 or indo-1, no lower limit was found for the 95% CI.