Table 1.

Structural statistics for the NMR assignments and solution structures of the apo and Zn²⁺-bound monomeric CARD9–CARD

	Apo CARD	Zn2+-bound CARD
PDB code	6E26	6E25
BMRB code	30492	30491
Assignments (%)a	89 (97)	81 (77)
1H	91 (97)	90 (97)
13C	91 (98)	72 (49)
15N	76 (96)	75 (95)
NOE restraints	1509	1592
Intra-residue (i = j)	423	407
Sequential (|i − j| = 1)	410	421
Medium range (|i − j| <5)	395	444
Long range (|i − j| ≥5)	281	320
NOE constraints per restrained residueb	15.9	16.8
Hydrogen bond constraints	66	60
Long range (|i − j| ≥5)	0	0
Dihedral angle constraints	140	140
Total no. of restricting constraints	1715	1792
Restricting constraints per restrained residueb	18.1	18.9
Long range (|i − j| ≥5)	3.0	3.4
Total structures computed	100	100
No. of structures included	20	20
Distance violations per structure
0.1–0.2 Å	9.0	6.4
0.2–0.5 Å	2.0	1.25
>0.5 Å	0	0
RMS of distance violation/constraint (Å)	0.02	0.02
Maximum distance violation (Å)	0.47	0.46
Dihedral angle violations per structure
1–10°	4.1	2.55
>10°	0	0
RMS of dihedral angle violation per constraint (°)	0.52	0.33
Maximum dihedral angle violation (°)	7.1	4.6
RMSDc
Backbone	0.7 (1.8)	0.5 (1.5)
Heavy atoms	1.2 (2.2)	1.0 (1.9)
Ramachandrand
Most favored (%)	93.6	94.9
Additionally allowed (%)	6.4	5.0
Generously allowed (%)	0	0.1
Disallowed	0	0

^a Total assignment completeness, with backbone completeness reported in parentheses.

^b Residues 3–97 contain conformational restraining constraints.

^c Residues 10–97 reported, with all-residue RMSDs reported in parentheses.

^d Residues 10–97, calculated with Procheck.