Table 2.
Structural statistics for the CARD9 CARD apo and Zn2+-bound domain-swapped dimer crystal structures
| Apo CARD dimer | Zn2+-bound CARD dimer | |
|---|---|---|
| PDB code | 6E28 | 6E27 |
| Wavelength | 1.000 | 1.000 |
| Space group | P 1 21 1 | P 1 21 1 |
| Cell dimensions | ||
| a, b, c (Å) | 43.98 37.43 56.88 | 44.01 37.46 56.96 |
| α, β, γ (°) | 90 101.47 90 | 90 101.79 90 |
| Resolution range (Å) | 37.94–1.36 (1.41–1.36) | 43.08–1.81 (1.88–1.81) |
| Rmerge (%) | 3.905 (84.11) | 7.536 (120) |
| I/σI | 21.67 (1.71) | 14.06 (1.37) |
| CC1/2 | 0.999 (0.715) | 0.999 (0.540) |
| Completeness (%) | 95.72 (73.23) | 98.24 (95.65) |
| Redundancy | 6.3 (5.2) | 6.6 (6.6) |
| Resolution (Å) | 1.36 | 1.81 |
| Unique reflections | 37,560 (2849) | 16,495 (1582) |
| Rwork/Rfree (%) | 0.1797 / 0.1937 | 0.2066 / 0.2577 |
| Non-hydrogen atoms | ||
| Protein | 1524 | 1498 |
| Ligands | 0 | 1 |
| Water | 153 | 89 |
| Average B-factor (Å2) | ||
| Protein | 36.48 | 44.78 |
| Ion | 63.81 | |
| Water | 41.34 | 45.46 |
| RMSD | ||
| Bond length (A) | 0.009 | 0.011 |
| Bond angles (°) | 1.01 | 1.05 |
| Ramachandran | ||
| Favored (%) | 99.45 | 98.89 |
| Allowed (%) | 0.55 | 1.11 |
| Outliers (%) | 0 | 0 |