Figure 4.

Mutation of J3 and HSP90 chaperones affects membrane association of AGO1. A and B, Western blotting of AGO1, J2/J3, HSP70, and HSP90 in total and microsome fractions prepared from inflorescence lysates of the indicated genotypes. For total fractions, equal loading was verified by Coomassie staining (CBB); for microsome fractions, Western blots were probed with SIP2 antibodies. Different sections of the membranes used for analysis of total lysates and microsome fractions were used for verification of protein loading; the leftmost molecular weight standard refers to the membrane used for total lysates, whereas the rightmost molecular weight standard refers to the membrane used for microsome fractions.