Figure 7.

Membrane-bound RISC is loaded in the absence of J2/J3 farnesylation. A, AGO1 immunoprecipitates from soluble and membrane fractions analyzed by small RNA Northern blots. An aliquot of the immunoprecipitate was used for AGO1 Western blots to document equal recovery of the AGO1 protein from the different samples. The same Northern membrane was used for consecutive hybridizations to all probes. B, small RNA-seq analysis of small RNA isolated from AGO1 immunoprecipitates from microsome fractions. Ratios of read counts of pairs of miRNA/miRNA* are depicted for the indicated genotypes.