Figure 6.

R388 CF in the presence of increasing concentrations of 2-bromopalmitic acid. Bacterial conjugation experiments were performed by a fluorescence-based assay as described under “Experimental procedures.” A, values represent the mean CF ± S.D. from at least four independent experiments relative to a positive control in the absence of inhibitor (100%). B, the relative CF from individual experiments in the presence of 2-bromopalmitic acid (50 μm, black circles) and a mixture of both palmitic acid (Palm, 500 μm) and 2-bromopalmitic acid (50 μm) (gray squares). Horizontal and vertical bars represent the mean ± S.D. of each group of data. Statistical significance was analyzed by Student's t test (**, p < 0.05).