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N-Glycan composition of the recombinant Fc γ receptors. Elution pattern and glycan composition of N-glycans from recombinant Fc γ receptors using hydrophilic interacting chromatography–LC/MS/MS are shown. A–D, expression of receptors using HEK293F cells provides primarily CT N-glycans (A and B) and Man5 N-glycans (C and D) results from expression using HEK293S (Gnt1-) cells. The numbers in the pie charts indicate the percentages of the N-glycan groups.
