Table 1.
cGMP-dependent phosphotransferase activity
| Constructs | Reduced |
Oxidized |
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Vmina | Vmaxa | Vmax/Vmin | Kab | nHb | n | Vmina | Vmaxa | Vmax/Vmin | Kab | nHb | n | |
| μmol/min × mg | μmol/min × mg | μm | μmol/min × mg | μmol/min × mg | μm | |||||||
| PKG Iα | ||||||||||||
| WT | 0.22 ± 0.00 | 4.39 ± 0.03 | 20.0 | 0.20 ± 0.00 | 1.7 ± 0.0 | 12 | 1.13 ± 0.08 | 2.77 ± 0.03 | 2.5 | NA | NA | 16 |
| C42S | 0.32 ± 0.06 | 2.31 ± 0.18 | 7.3 | 0.58 ± 0.03 | 1.1 ± 0.1 | 14 | 1.10 ± 0.03 | 2.36 ± 0.11 | 2.1 | 0.13 ± 0.01 | 1.6 ± 0.2 | 6 |
| Δ53 | 0.18 ± 0.16 | 3.95 ± 0.30 | 21.6 | 0.22 ± 0.03 | 0.9 ± 0.1 | 7 | 1.38 ± 0.19 | 1.58 ± 0.36 | 1.1 | NA | NA | 6 |
| C117S | 0.16 ± 0.10 | 4.09 ± 0.13 | 26.0 | 0.12 ± 0.01 | 1.4 ± 0.1 | 6 | 0.65 ± 0.14 | 3.81 ± 0.10 | 5.9 | 0.20 ± 0.00 | 1.2 ± 0.1 | 6 |
| C195S | 0.43 ± 0.02 | 4.67 ± 0.12 | 10.9 | 0.27 ± 0.01 | 1.8 ± 0.1 | 6 | 0.86 ± 0.02 | 3.20 ± 0.05 | 3.7 | 1.22 ± 0.11 | 0.8 ± 0.1 | 6 |
| PKG Iβ | ||||||||||||
| WT | 0.04 ± 0.01 | 3.62 ± 0.26 | 86.1 | 1.21 ± 0.03 | 1.9 ± 0.1 | 7 | 0.42 ± 0.03 | 2.78 ± 0.10 | 6.6 | 0.86 ± 0.03 | 1.2 ± 0.1 | 6 |
a The velocity values indicate the μmol of peptide substrate (W15) phosphorylated/min/mg of PKG (μmol/min × mg) and are represented as the mean ± S.D.
b Values were obtained from non-linear regression fit analyses and are represented as the means ± standard deviations. Not applicable (NA) values indicate the data for that condition could not be fit to a non-linear regression.