Figure 11.

Comparison of the active sites of CMMase and TpCADE. A, CMMase (green)–CMM (gray) complex structure. B, TpCADE (cyan)–CNN (black) complex structure (PDB 5I0G). The structures were superimposed with the following atoms and presented side-by-side: side chain oxygen atoms of the catalytic residues (nucleophile and acid/base catalyst), the scissile glycosidic bond oxygen, and the sugar ring atoms in subsite −1. Hydrogen bonds and the nucleophilic attack distances are shown as yellow and red dashed lines, respectively.