Figure 4.

Effects of EMB on the transcriptional activity and expression level of EtbR. A, β-gal activity assays for regulation of EtbR in the absence (No drug) or presence of EMB. The reporter cassettes are identical to that in Fig. 2. Different significances of indicated groups were calculated and are shown by p value and asterisks. B, qRT-PCR assays for inhA expression patterns in M. tuberculosis WT strain under various concentrations (0–1 μg/ml) of EMB for 24 h. The data were analyzed as in Fig. 2. Error bars, S.D. of replicates. Asterisks represent significant difference (***, p < 0.001, two-tailed Student's t test) between two groups.