Table 2.
Kinetic parameters for fTHP hydrolysis at 23 °C
Hydrolysis occurs at the Gly–Leu bond as monitored by MALDI-TOF-MS.
| Enzyme | Peptide | kcat/Km | kcat | Km |
|---|---|---|---|---|
| s−1 m−1 | s−1 | μm | ||
| MMP-1 | fTHP-15b | 1,153 ± 120 | 0.0054 ± 0.0004 | 4.72 ± 0.73 |
| MMP-1 | Ile–Tyr fTHPb | 2705 ± 212 | 0.0112 ± 0.0020 | 4.18 ± 1.07 |
| MMP-1 | Ile–Lys fTHPb | 361 ± 18 | 0.0040 ± 0.0008 | 11.0 ± 1.93 |
| MMP-1 | Asp–Lys fTHPb | 2,466 ± 417 | 0.0109 ± 0.0017 | 4.41 ± 0.06 |
| MMP-1 | Trp–Lys fTHPb | 2,936 ± 234 | 0.0468 ± 0.0020 | 16.0 ± 0.58 |
| MMP-1 | fTHP-18 | 1,850 ± 50 | 0.0140 ± 0.0017 | 7.56 ± 1.11 |
| MMP-1 | fTHP-18 Ile–Tyr | 2,972 ± 151 | 0.00957 ± 0.00072 | 3.22 ± 0.08 |
| MMP-2 | fTHP-15b | 81,222 ± 21,453 | 0.132 ± 0.012 | 1.67 ± 0.30 |
| MMP-2 | Ile–Tyr fTHPb | 47,989 ± 7,430 | 0.099 ± 0.028 | 2.05 ± 0.39 |
| MMP-2 | Ile–Lys fTHPb | 64,631 ± 7,402 | 0.267 ± 0.029 | 4.16 ± 0.43 |
| MMP-2 | Asp–Lys fTHPb | 48,426 ± 3,382 | 0.453 ± 0.020 | 9.40 ± 1.01 |
| MMP-2 | Trp–Lys fTHPb | 139,415 ± 14,032 | 0.206 ± 0.019 | 1.48 ± 0.13 |
| MMP-2 | fTHP-18 | 80,444 ± 20,837 | 0.124 ± 0.010 | 1.60 ± 0.37 |
| MMP-2 | fTHP-18 Ile–Tyr | 24,487 ± 1,918 | 0.127 ± 0.022 | 5.26 ± 1.22 |
| MMP-8 | fTHP-15b | 2,567 ± 443 | 0.0345 ± 0.0018 | 13.6 ± 1.57 |
| MMP-8 | Ile–Tyr fTHPb | 4,297 ± 97 | 0.108 ± 0.0009 | 25.1 ± 0.35 |
| MMP-8 | Ile–Lys fTHPb | 2,502 ± 358 | 0.0900 ± 0.0160 | 36.2 ± 6.45 |
| MMP-8 | Asp–Lys fTHPb | 2,383 ± 219 | 0.0780 ± 0.0097 | 32.7 ± 1.42 |
| MMP-8 | Trp–Lys fTHPb | 3,702 ± 666 | 0.101 ± 0.007 | 27.6 ± 3.07 |
| MMP-8 | fTHP-18 | 7,308 ± 970 | 0.331 ± 0.071 | 45.0 ± 3.74 |
| MMP-8 | fTHP-18 Ile–Tyr | 6,986 ± 193 | 0.218 ± 0.026 | 31.3 ± 4.63 |
| MMP-9 | fTHP-15b | 17,398 ± 689 | 0.0258 ± 0.0034 | 1.48 ± 0.15 |
| MMP-9 | Ile–Tyr fTHPb | 364 ± 121 | 0.00217 ± 0.00038 | 6.55 ± 2.78 |
| MMP-9 | Ile–Lys fTHPb | 8,005 ± 1,081 | 0.0176 ± 0.0010 | 2.22 ± 0.34 |
| MMP-9 | Asp–Lys fTHPb | 1,811 ± 474 | 0.00380 ± 0.00156 | 2.11 ± 0.67 |
| MMP-9 | Trp–Lys fTHPb | 3,246 ± 701 | 0.0331 ± 0.0066 | 10.5 ± 2.99 |
| MMP-9 | fTHP-18 | 29,869 ± 2,662 | 0.0934 ± 0.0058 | 3.14 ± 0.30 |
| MMP-9 | fTHP-18 Ile–Tyr | 19,798 ± 1,277 | 0.0338 ± 0.0012 | 1.72 ± 0.17 |
| MMP-13 | fTHP-15b | 57,785 ± 5,337 | 1.056 ± 0.227 | 18.4 ± 4.75 |
| MMP-13 | Ile–Tyr fTHPb | 56,486 ± 12,480 | 0.115 ± 0.003 | 2.12 ± 0.56 |
| MMP-13 | Ile–Lys fTHPb | 43,605 ± 3,016 | 0.448 ± 0.009 | 10.3 ± 0.50 |
| MMP-13 | Asp–Lys fTHPb | 13,187 ± 2,173 | 0.216 ± 0.017 | 16.7 ± 4.01 |
| MMP-13 | Trp–Lys fTHPb | 65,818 ± 7,145 | 0.527 ± 0.041 | 8.06 ± 0.91 |
| MMP-13 | fTHP-18 | 14,151 ± 645 | 0.257 ± 0.023 | 18.2 ± 2.23 |
| MMP-13 | fTHP-18 Ile–Tyr | 51,870 ± 8,370 | 0.247 ± 0.048 | 4.75 ± 0.15 |
| MMP-14 | fTHP-15b | 7,766 ± 399 | 0.0367 ± 0.0121 | 4.72 ± 1.49 |
| MMP-14 | Ile–Tyr fTHPb | 12,639 ± 720 | 0.243 ± 0.011 | 19.2 ± 1.42 |
| MMP-14 | Ile–Lys fTHPb | 11,861 ± 2,570 | 0.218 ± 0.021 | 18.6 ± 2.30 |
| MMP-14 | Asp–Lys fTHPb | 6,314 ± 1,676 | 0.0434 ± 0.0302 | 6.81 ± 3.69 |
| MMP-14 | Trp–Lys fTHPb | 5,318 ± 434 | 0.0252 ± 0.0019 | 4.79 ± 0.74 |
| MMP-14 | fTHP-18 | 25,720 ± 3,021 | 0.0920 ± 0.0138 | 3.65 ± 0.91 |
| MMP-14 | fTHP-18 Ile–Tyr | 10,845 ± 1,817 | 0.0865 ± 0.0192 | 7.94 ± 0.44 |
| MMP-15 | fTHP-15b | 22,073 ± 3,210 | 0.0815 ± 0.0462 | 3.89 ± 2.66 |
| MMP-15 | Ile–Tyr fTHPb | 8,264 ± 2,030 | 0.0884 ± 0.0534 | 10.4 ± 5.55 |
| MMP-15 | Ile–Lys fTHPb | 6,299 ± 365 | 0.185 ± 0.001 | 29.4 ± 1.61 |
| MMP-15 | Asp–Lys fTHPb | 686 ± 39 | 0.0206 ± 0.0055 | 24.9 ± 0.62 |
| MMP-15 | Trp–Lys fTHPb | 7,076 ± 216 | 0.111 ± 0.019 | 15.7 ± 2.17 |
| MMP-15 | fTHP-18 | 2,884 ± 163 | 0.123 ± 0.024 | 43.0 ± 10.6 |
| MMP-15 | fTHP-18 Ile–Tyr | 7,377 ± 1,795 | 0.112 ± 0.008 | 15.7 ± 3.10 |