Table 3.
Tm values obtained for the fTHP-branched mixtures and individual peptides
| Peptide | Tm | Peptide | Tm |
|---|---|---|---|
| °C | °C | ||
| Pro P10′ fTHPbm | 38.6 | Hyp P11′ fTHPbm | 49.8 |
| Gly P10′ fTHPbm | 25.2 | Gly P11′ fTHPbm | 38.2 |
| Ala P10′ fTHPbm | 31.8 | Ala P11′ fTHPbm | 43.8 |
| Val P10′ fTHPbm | 27.4 | Val P11′ fTHPbm | 44.2 |
| Leu P10′ fTHPbm | 37.2 | Leu P11′ fTHPbm | 40.4 |
| Ile P10′ fTHPbm | 40.4 | Ile P11′ fTHPbm | 34.8 |
| Phe P10′ fTHPbm | 39.2 | Phe P11′ fTHPbm | 25.2 |
| Met P10′ fTHPbm | 31.2 | Met P11′ fTHPbm | 32.2 |
| Asn P10′ fTHPbm | 38.8 | Asn P11′ fTHPbm | 31.0 |
| Gln P10′ fTHPbm | 37.5 | Gln P11′ fTHPbm | 36.6 |
| His P10′ fTHPbm | 29.6 | His P11′ fTHPbm | ∼21 |
| Lys P10′ fTHPbm | 33.2 | Lys P11′ fTHPbm | 28.8 |
| Arg P10′ fTHPbm | 34.2 | Arg P11′ fTHPbm | 36.2 |
| Trp P10′ fTHPbm | 35.6 | Trp P11′ fTHPbm | 28.2 |
| Asp P10′ fTHPbm | 28.4 | Asp P11′ fTHPbm | 42.2 |
| Glu P10′ fTHPbm | 38.6 | Glu P11′ fTHPbm | 39.8 |
| Ser P10′ fTHPbm | 41.6 | Ser P11′ fTHPbm | 37.8 |
| Thr P10′ fTHPbm | 37.2 | Thr P11′ fTHPbm | 36.2 |
| Tyr P10′ fTHPbm | 33.8 | Tyr P11′ fTHPbm | 33.0 |
| fTHP-15b (crude) | 37.8 | fTHP-15b (purified) | 44.5 |
| Ile–Lys fTHPb (crude) | 26.8 | Ile-Lys fTHPb (purified) | 26.0 |
| Ile–Tyr fTHPb (crude) | 26.6 | Ile-Tyr fTHPb (purified) | 28.4 |
| Asp–Lys fTHPb (crude) | 24.0 | Asp-Lys fTHPb (purified) | 25.6 |
| Trp–Lys fTHPb (crude) | 35.8 | Trp-Lys fTHPb (purified) | 39.2 |
| fTHP-18 (purified) | 45.4 | fTHP-18 Ile-Tyr (purified) | 36.6 |