Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Sep 13;293(43):16697–16708. doi: 10.1074/jbc.RA118.003781

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018 Yao et al.

Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

Figure 2. — WW2 cooperates with L to regulate Su(dx) activity by binding to HECT. A, analytical gel filtration assay showing that MBP-tagged WW12L34 can form a stable complex with MBP-HECT. The elution profile of globular markers is indicated with a dashed gray line. B, the isolated L could not form a complex with HECT. C, WW12L34 can inhibit the autoubiquitination of Su(dx) HECT in a dose-dependent manner. D, Su(dx) HECT autoubiquitination assay with different concentrations of MBP-L. E and F, analytical gel filtration assay showing that WW12L (E) but not LWW34 (F) can form a stable complex with HECT. G and H, Su(dx) HECT ubiquitination assay in the presence of WW12L (G) or LWW34 (H). For the ubiquitination assay, reactions were quenched at 5 or 10 min. Data are presented as the mean ± S.D. (error bars) of triplicate experiments; ns, not significant; ***, p < 0.001; ****, p < 0.0001 using two-way analysis of variance with Dunnett's multiple-comparison test. Asterisks, MBP- or GST-tagged HECT.