Figure 3.

Nr2f6^−/− CD4⁺ T cells do not induce enhanced colitis in Rag1^−/− mice. (A) Body weight, (B) Kaplan-Meier survival curve and (C) colon length of Rag1^−/− recipient mice receiving adoptive cell transfer of 5×105 naive (CD25^-CD62L^hiCD44^lo) CD4⁺ T cells isolated from peripheral lymphatic organs of wild-type or Nr2f6^−/− mice (n=15). (D) Representative H&E-stained colonic Swiss rolls, (E) histological severity scores and (F) epithelial thickness of Rag1^−/− recipients adoptively transferred with either wild-type or Nr2f6^−/− naive CD4⁺ T cells (nCD4⁺). Data are presented as mean±SEM error bars and are representative of at least two independent experiments. Unpaired Student’s t-test, * p<0.05.