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. 2018 Apr 5;73(8):758–768. doi: 10.1136/thoraxjnl-2017-210326

Figure 1.

Figure 1

Nucleotide-binding domain, leucine-rich-containing family, pyrin-domain-containing-3 (NLRP3) inflammasome is involved in Aspergillus fumigatus (Af)-induced allergic lung inflammation. (A and B) Representative confocal images of bronchoalveolar lavage (BAL) cells show the localisation of NLRP3 (red), caspase-1 (Casp-1, green) and apoptosis-associated speck-like protein containing a carboxy-terminal caspase-recruitment domain (ASC; green) from saline-exposed (control) or Af-exposed (Af) mice. 4',6-Diamidino-2-phenylindole (DAPI) stain was used for nuclear localisation. Bars indicate 20 µm. (C) Representative immunoblots and densitometric analyses of IL-1β in BAL fluids from saline-exposed (control) and Af-exposed (Af) mice. Bars represent mean±SEM from six mice per group. # P<0.05 versus control. (D) Representative confocal images show the localisation of NLRP3 (red) and Casp-1 (green) in lung tissues from saline-exposed (control) and Af-exposed (Af) mice. Bars indicate 50 µm. (E) Representative immunoblots and densitometric analyses of IL-1β in lung tissues from saline-exposed (control) and Af-exposed (Af) mice. Bars represent mean±SEM from six mice per group. # P<0.05 versus control. (F) Representative confocal image for NLRP3 in lung tissues from a healthy control, a patient with idiopathic pulmonary fibrosis (IPF; disease control), and a patient with allergic bronchopulmonary aspergillosis (ABPA), respectively. Bars indicate 50 µm. (G) Representative immunohistochemical staining of NLRP3 in lung tissues of a healthy person, a patient with IPF and a patient with ABPA. Brown-stained cells were considered to express the NLRP3 protein. Bars indicate 20 µm.