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. 2018 Aug 27;7(11):e1507668. doi: 10.1080/2162402X.2018.1507668

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© 2018 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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Figure 7. — Dex-treatment suppresses expression of myeloid markers in vitro. Monocyte derived macrophages were treated with 0.1, 1, 10 μM Dex for 24 hours, then detached and immunostained for (A) HLA-DR, DP, DQ (MHC-II), (B) CD86, (C) CD80, (D) CD40, (E) HLA-ABC (MHC-I), (F) CCR2 (G) PD-L1, and (H) CD163 and analyzed by flow cytometry. The background mean fluorescence intensity (MFI) of unstained samples was subtracted from experimental samples. Data was then expressed as the fold of treated MFI over untreated (control) MFI. P-values calculated with a one-way ANOVA followed by Dunnett’s multiple comparisons test comparing treated samples to control. Line indicates mean, error bars indicate SEM.