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. 2018 Aug 24;7(11):e1509821. doi: 10.1080/2162402X.2018.1509821

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© 2018 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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Figure 1. — H6 upregulates phosphorylation of STAT3 transcription factor in AGI-101H cell lines Mich1 and Mich2 that elicit expression of melanoma-initiating cell markers ALDH1, CD271, ABCB5, as well as a microphthalmia-associated transcription factor (MITF). A. Flow cytometry analysis of STAT3-pY705 and STAT3-pS727 modifications in Mich1 and Mich2 cell lines as well as their Hyper-IL6-modified derivatives. B. Flow cytometry analysis of ALDH1 activity demonstrated with Aldefluor assay. C and D. Flow cytometry analysis of CD44 (C) and CD271 (D) expression on the surface of Mich1 and Mich2 cell lines as well as their Hyper-IL6-modified derivatives. E. Western blot analysis of ABCB5 marker. F. Western blot analysis of MITF. For Western Blot assays, GAPDH was used as an internal control.