Figure 1.

Generation of primary human MC.

(a) Granule development in CD34⁺ derived human MC during the course of culture (scale bar, 20μm). (b) Viability and purity of human MC followed by dead cell removal and CD117⁺ positive selection. (c) β-hexosaminidase (β-hex) release by human MC in response to FcɛRI-mediated activation. Bars represent mean value ± SEM. Data shown are a representative of three independent experiments.