Figure 4.

Crosstalk between human MC and colon cancer cells is necessary for cancer proliferation.

Proliferation percentage (% proliferation) of colon cancer cells (HT29 and Caco2) cultured with MC directly or MC supernatant alone in a serum-free medium for 72 hr. The proliferation of cancer cells cultured in serum-free medium alone serves as a negative control (NEG) and in FCS-containing medium serves as a positive control (POS). Proliferation percentage was calculated as follows: BrdU uptake [(treated group - NEG)/(POS – NEG)] x 100%. Data are displayed as mean ± SEM of three independent experiments (n=3). *P ˂ 0.05; **P ˂ 0.005; ***P ˂ 0.001, assessed by one-way ANOVA.