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. 2018 Oct 12;27(20):1412–1425. doi: 10.1089/scd.2018.0071

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Copyright 2018, Mary Ann Liebert, Inc., publishers

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FIG. 6. — Deleted Ihh in the mesenchyme promotes osteogenic differentiation and mineralization in vitro. (A) GFP-positive cells isolated from P6 murine costal tissue. (B) Flow cytometry analysis confirmed that majority of the costal cells differentiate from deleted Ihh/EGFP-positive mesenchyme cells. (C) Alizarin red and von Kossa staining indicates the presence of a mineralizing extracellular matrix over time in 3 weeks of culture under no osteogenesis induction condition. (D) Real-time PCR shows deleted Ihh in the limb mesenchyme increases bone formation marker genes. **P < 0.01, *P < 0.05.

FIG. 6. — Deleted Ihh in the mesenchyme promotes osteogenic differentiation and mineralization in vitro. (A) GFP-positive cells isolated from P6 murine costal tissue. (B) Flow cytometry analysis confirmed that majority of the costal cells differentiate from deleted Ihh/EGFP-positive mesenchyme cells. (C) Alizarin red and von Kossa staining indicates the presence of a mineralizing extracellular matrix over time in 3 weeks of culture under no osteogenesis induction condition. (D) Real-time PCR shows deleted Ihh in the limb mesenchyme increases bone formation marker genes. **P < 0.01, *P < 0.05.