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. 2018 Oct 5;9(78):34821–34837. doi: 10.18632/oncotarget.26201

Figure 6. PARP1−/− cells are severely compromised in surviving telomeric stress.

Figure 6

(A) growth curves plotting population doublings (PD) and days in culture. Each line represents an independent subclone. For some of the wild type clones the experiment was intentionally terminated after 80 days and for others only after 120 days. (B) growth curves plotting PD and days in culture for PARP1-null cells; only a single subclone (green line) survived beyond 40 days. (C) Top. STELA of the 17p telomere. The PD from the point of single cell cloning is shown above, while the mean (in kb) of the telomere length profiles is shown below. Bottom. Single-molecule telomere fusion analysis, using oligonucleotide PCR primers targeted to XpYp, 17p and the 21q family of related telomeres at the PD points as indicated.