Figure 6. H19 contributes to cell adhesion through regulation of CD24 and integrin expression in PDAC cells.
(A and B) Adhesion assays were performed using mock and H19-overexpressing cells (A) or Sc and H19-knockdown cells (B). Each cell type exhibited negligible adhesion to BSA. **P < 0.01. (C and D) qRT-PCR analysis of integrins was performed using cDNA derived from mock and H19-overexpressing cells (C) or Sc and H19-knockdown cells (D). Results are presented as means ± SD from three independent experiments. **P < 0.01. (E and F) Flow-cytometry analysis of integrin and CD24 levels in mock and H19-overexpressing cells (E) or Sc and H19-knockdown cells (F). Controls are indicated by thin lines with gray color. MFIs relative to mock or Sc cells are shown on the right side. Results are presented as means ± SD from three independent experiments. *P < 0.05, **P < 0.01.