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. Author manuscript; available in PMC: 2018 Oct 29.
Published in final edited form as: J Immunol Tech Infect Dis. 2016 May 10;5(3):10.4172/2329-9541.1000146. doi: 10.4172/2329-9541.1000146

Figure 3.

Figure 3

ROS production and activation by PMNs associated with mCherry-Li and “bystander” uninfected neutrophils. (A) Flow plots from a 30 minute infection of human neutrophils with opsonized mCherry-Li. (B) shows the expression of DHR123 in infected PMNs, indicating the amounts of ROS generated. PMNs were incubated with either mCherry-Li at the indicated MOI, or buffer, or PMN supernatant for 10 minutes at 37°C. DHR123 was then added to cultures, and after an additional 20 minutes at 37°C, cells were fixed and stained for flow cytometry. PMN supernatant was collected from a previous PMN infection (MOI 5:1, 30 minutes). (C) Flow cytometry plots of surface CD62L and DHR123 in mCherry+ or mCherry− PMNs incubated for 30 minutes with mCherry-Li at 37°C is shown. Negative and positive controls were PMNs incubated with buffer, or with opsonized zymosan (OZ), respectively.