Figure 3. The effect of ET-1 (100 nM) on Ca²⁺ transients in cytoplasmic and perinuclear regions of CMs isolated from WT (A) or PLB-KO mice (B).

a. representative traces of Ca²⁺ transients in cytoplasmic and perinuclear (between red lines) regions of CMs. b and c, intensity profiles and biophysical parameters of Ca²⁺ transients in cytoplasmic and perinuclear regions of CMs. Each Ca transient have its own diastolic Ca level diastolic Ca in the absence of ET was used for F0 determination. * indicates p<0.05 vs control (average of 10 CMs from 5 WT or 10 CMs from 5 PLB KO mice, respectively).