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. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: J Mol Cell Cardiol. 2018 Sep 24;123:185–197. doi: 10.1016/j.yjmcc.2018.09.008

Fig. 8. Effects of anti-PLB Fab on Ca2+ concentration in the lumen of NE and SR.

Fig. 8.

Permeabilized CMs or isolated cardiac nuclei from both WT and PLB-KO mice were loaded with mag-fluo-4 and imaged. A. Representative 2D confocal images of mag-fluo-4 signals in the NE and SR from permeabilized dog CMs at a. baseline; b. after addition of IP3 (10 μM); and c. sequential addition of anti-PLB Fab (100 μg/ml), and IP3 (10 μM). d. graphs show time-dependent intensity profiles (F/F0min) after treatments. n= 6 CMs from 2 dogs. B. Representative 2D confocal images of mag-fluo-4 signals in the NE in isolated cardiac nuclei from WT and PLB-KO mice at control (Ctl) and after addition of anti-PLB Fab (Fab). Bar graph shows the mag-fluo-4 intensity ratios after addition of anti-PLB Fab.