Table 1.
Primer sequences for the characterization of the canine mammary gland tumor (CMT) adenocarcinoma cell.
| Genes | Primer Sequence (5' – 3') | Annealing Temperature (°C) |
|---|---|---|
| Cytokeratin-8 (CK-8) |
Forward: AAGCAGCTTCTCCGCTCCTTCTA Reverse: CCAGCTTCAGTTTCTCCTAGCCCA |
50 |
|
| ||
| Hypoxanthine ribosyltransferase (HPRT) |
Forward: TTA TCA GAC TGA AGA GCT ACT Reverse: TTA CCA GTG TCA ATT ATA TCT TCA ACA ATC |
60 |
|
| ||
| Estrogen Receptor (ER) |
Forward: TCAGCTCTCGTCCTTCCTGCA Reverse: ACCAGACTCCATAATGGTAGCCTG |
56 |
|
| ||
| Progesterone Receptor (PGR) |
Forward: AGGTGTACCAGCCGTACCTCAA Reverse: TTCGACCTCCAAGGACCATGCCAG |
56 |
|
| ||
| Vascular Endothelial Growth Factor Receptor (VEGF) |
Forward: GCTCTCTTGGGTGCATTGGAGC Reverse: TAGGCCCTCATCATTACAGCAGC |
58 |
|
| ||
| Human epidermal growth factor receptor 2 (HER2) | Forward: ACCGGCACAGACATGAAGCTCC Reverse: AGCGGGTCTCCATTGTCCAGCA |
50 |
|
| ||
| Hypoxia-inducing Factor-1α (HIF-1α) |
Forward: CGTTCCTTCGATCAGTTGTC Reverse: TCAGTGGTGGCAGTGGTAGT |
56 |
|
| ||
| B-cell lymphoma-2 (Bcl-2) |
Forward: ATGTGTGTGGAGAGCGTCAACC Reverse: TGAGCAGAGTCTTCAGAGACAGCC |
50 |
|
| ||
| Matrix Metallopeptidase-2 (MMP-2) |
Forward: CCACGTGACAAGCCCATGGGGCCCC Reverse: GCAGCCTAGCCAGTCGGATTTGATG |
60 |
|
| ||
| Erythropoietin Receptor (EPOR) |
Forward: CCTGACGCTCTCCCTCATCC Reverse: GCCTTCAAACTCGCTCTCTGG |
65 |