Figure 3.
Overexpression of GAS5 Inhibits Cell Growth, Migration, and Chemotherapy Resistance In Vitro
(A) RT-PCR of GAS5 expression after transfection with a GAS5 overexpression vector. Data are means ± SD (n = 3). ***p < 0.001 versus control group. (B) Cell proliferation was examined by CCK8 assays. Data are means ± SD. *p < 0.05 and ***p < 0.001 versus control. (C) The relative migration cells were analyzed. (D) Transwell analyses showed the effects of GAS5 on PANC-1 cell migration (magnification, 200×). Data are means ± SD. ***p < 0.001 versus control. (E) Comparison of cell viability and IC50 of gemcitabine in PANC-1 cells with or without GAS5 overexpression. (F) Effect of GAS5 on gemcitabine-induced apoptosis detected using annexin V-PI double staining after exposure to 10 nM gemcitabine for 72 hr. (G) The relative apoptosis cells were calculated. Data are means ± SD. ***p < 0.001 versus control.